In vitro changes in back vertex distance of chick and pigeon lenses: Species differences and the effects of aging

We have used a scanning laser technique to measure in vitro changes in back vertex distance of chick and pigeon lenses. Enucleated eyes were dissected, leaving the lens naturally suspended by the ciliary body and intraocular muscles. Ray tracing techniques were used to measure the resting back vertex distance of the lenses by passing a laser beam through the lens and scanning it across the pupillary aperture. The pupil diameter was measured videographically. The measurements were repeated while the intraocular muscles were pharmacologically stimulated with increasing concentrations of either nicotine or carbachol. Drug stimulation caused changes in pupil diameter and changes in the back vertex distance of the lenses. These experiments were conducted on the eyes of young chicks, young pigeons, and on the eyes of three pigeons older than 10 yr. The lenses from the eyes of the old pigeons had the greatest resting back vertex distance, followed by those of the young pigeons and finally those of the young chicks. Lenses from the eyes of young chicks and young pigeons underwent similar drug-induced changes in back vertex distance, but the lenses from old pigeon eyes showed an almost complete absence of such changes. Further, we demonstrated that, just as in the chick eye, lenticular changes in pigeon eyes are due to a contraction of the iris muscle. This is evident because after the iris has been removed the lens undergoes no change in back vertex distance during stimulation. We conclude with a discussion of the lenticular accommodative ability of the pigeon eye with reference to the recently reported accommodative mechanism of the chick eye and a comparison of chick and pigeon iris morphology.